Reagentless, Structure-Switching, Electrochemical Aptamer-Based Sensors
نویسندگان
چکیده
منابع مشابه
Aptamer based electrochemical sensors for emerging environmental pollutants
Environmental contaminants monitoring is one of the key issues in understanding and managing hazards to human health and ecosystems. In this context, aptamer based electrochemical sensors have achieved intense significance because of their capability to resolve a potentially large number of problems and challenges in environmental contamination. An aptasensor is a compact analytical device inco...
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Electrochemical aptamer-based (E-AB) sensors have emerged as a promising and versatile new biosensor platform. Combining the generality and specificity of aptamer-ligand interactions with the selectivity and convenience of electrochemical readouts, this approach affords the detection of a wide variety of targets directly in complex, contaminant-ridden samples, such as whole blood, foodstuffs an...
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Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as approximately 3 nM.
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ژورنال
عنوان ژورنال: Annual Review of Analytical Chemistry
سال: 2016
ISSN: 1936-1327,1936-1335
DOI: 10.1146/annurev-anchem-071015-041446